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Biologists are almost certain that eukaryotes evolved from prokaryotes because:
1. Both use RNA and DNA are the genetic material
2. Both use the same 20 amino acids
3. Both have ribosomes and DNA and RNA
4. Both have a lipid bilayer cell membrane.
5. Both use L amino acids and D sugars
Biologists are also almost certain that eukaryotes evolved only once (i.e., are monophyletic- descendants of a single common ancestor) because they all share:
1. microtubules (composed of the protein tubulin) and actin molecules-
cytoskeleton for support or intracellular transport. flagella (or cilia)
2. DNA in chromosomes (intertwined with histone protein)
3. membrane-bound organelles.
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